- ￥1380 - 2980
- Sino Biological
- 北京
- 50235-M08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse SerpinF1 / PEDF Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥1380.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥2980.0 |
蛋白名称：PEDF蛋白, PEDF protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Thr 417) of mouse SerpinF1 (NP_035470.3) precursor was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized mouse SERPINF1-His at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CSNK2A1 (Cat:10630-H09B) with a linear range of 0.31-2.5 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 20
蛋白分子量：The secreted recombinant mouse SerpinF1consists of 409 amino acids and has a calculated molecular mass of 45.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_035470.3
蛋白氨基酸序列：Met1-Thr417
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Blazejewski SM, et al. Rpsa Signaling Regulates Cortical Neuronal Morphogenesis via Its Ligand, PEDF, and Plasma Membrane Interaction Partner, Itga6.Cerebral cortex (New York, N.Y. : 1991), PubMed ID: 34347028
proliferation 、apoptosis 和 adhesion 方面的指标发现均与此无关。然后检测 integrins、junctional protein complexes、ECM proteins production 等其他的一些指标，并根据这些指标的结果做相关讨论，发现氧化应激和 extracellular PEDF 指标与 PRE 的迁移密切相关，至此，文章结束。大家是不是对这篇文章有个清晰的了解啦。对了，这个百度脑图可以自动保存在云端哦，如果想线下看这个线路图，还可以下载哦，通过「点击百度脑图
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
Mouse Strains and Genetic Nomenclature
., and Montagutelli, X. 2009. Genetic analysis of skull shape variation and morphological integration in the mouse using interspecific recombinant congenic strains between C57BL/6 and mice of the Mus spretus species. Evolution 63:2668‐2686
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