- ￥3870
- Sino Biological
- 北京
- 10351-H08E
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human HSP27 / HSPB1 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
蛋白名称：Hsp27/HSPB1蛋白, Hsp27/HSPB1 protein
蛋白构建：A DNA sequence encoding the human HSP27 (NP_001531.1) (Met1-Lys205) was expressed with a C-terminal polyhistidine tag.
表达宿主：E. coli
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：Please contact us for more information.
预测N端：Met 1
蛋白分子量：The recombinant human HSP27 consists of 211 amino acids and has a predicted molecular mass of 28 kDa as estimated by SDS-PAGE.
蛋白NP号：NP_001532.1
蛋白氨基酸序列：Met1-Lys205
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验StructureFunctions of HspB1 (Hsp27)
Human HspB1 (also denoted Hsp27) is a well-known member, together with alphaB-crystallin, of the small heat-shock (or stress) proteins (sHsps) (20–40 kDa). In this chapter, I describe procedures for testing the oligomeric and phosphorylation
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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