- 询价
- StressMarq
- 加拿大
- SPR-105
- 2025年11月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20ºC
- 保质期：
一年
- 英文名：
HSP27 Protein
- 库存：
大量
- 供应商：
StressMarq
- 规格：
50 µg/100 µg/100 µg x2
| 规格： | 50 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | 询价 |
| 规格： | 100 µg x2 | 产品价格： | 询价 |
| 产品名称 | HSP27 蛋白 |
| 产品描述 |
重组人HSP27蛋白 |
| 应用范围 | WB, SDS-PAGE, Functional Assay, ELISA |
| 浓度 | 各批次不同，请详见说明书 |
| 标记物 | His 标签 |
| 性质 | 重组 |
| 来源物种 | 人 |
| 表达系统 | 大肠杆菌 (E. coli) |
| 纯度 | >90% |
产品特性
| 储存缓冲液 | 50mM Tris/HCl, pH 8, 0.3M NaCl |
| 储存温度 | -20ºC |
| 运输温度 | 蓝冰或4℃ |
| 纯化方式 | 亲和纯化的 |
| Protein Size | 分子量约为27kD |
| 引用该产品 | Human Recombinant HSP27 Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-105) |
| 分析证书 | 该蛋白已经通过SDS-PAGE检测证明纯度大于90%. |
生物学特性
| 别名 | 28kDa heat shock Protein, CMT2F Protein, Hsp25 Protein, Hsp27 Protein, Hsp28 Protein, HspB1 Protein, SRP27 Protein |
| 研究领域 | 微丝, 心肌收缩, 肌动蛋白, 肌动蛋白组装, 伴侣蛋白, 微管, 心血管系统, 动脉粥样硬化, 心脏, 癌症, 细胞信号传导, 蛋白质运输, 细胞骨架, 肿瘤标记物 |
| 细胞定位 | 内质网腔, 细胞质, 黑素体 |
| Accession Number | BC012768 |
| GeneID | 3315 |
| Swiss Prot | P04792 |
| 科研背景 | HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermo tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6). Looking for more information on HSP27? Visit our new HSP27 Scientific Resource Guide. |
| 参考资料 | 1. Kim K.K., Kim R., and Kim, S. (1998) Nature 394(6693): 595-599. 2. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 3. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 4. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70. 5. Sarto C. Binnz P.A. and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26. 6. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6. |
产品图片
28kDa Hsp27 蛋白 SDS-PAGE (SPR-105).
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文献和实验[1] Lee S L, Dempsey-Hibbert N C, Vimalachandran D, et al. Re-examining HSPC1 inhibitors[J]. Cell Stress and Chaperones, 2017, 22(2): 293-306.
[2] Conway M, Nafar F, Straka T, et al. Modulation of amyloid-β protein precursor expression by HspB1[J]. Journal of Alzheimer's Disease, 2014, 42(2): 435-450.
植物源重组人胰岛素生长因子融合蛋白（OsrIGF-1），胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白，分子量7649Da，耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用，它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白（OsrIGF-1）在IGF-1的N末端融合了人血清白蛋白（HSA），HSA作为蛋白质保护剂增加了IGF
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人热休克蛋白27 ( HSP27 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 HSP27 单抗包被于酶标板上，标准品和样品中的 HSP27与单抗结合，加入生物素化的抗人 HSP27 ，形成免疫复合物连接在板上，辣根
由武汉大学生命科学院教授、武汉禾元生物科技有限公司董事长杨代常领衔的研发团队从2006年开始进行植物源替代血浆来源的医药蛋白的研究与开发，现已取得突破性进展并已跨入规模化生产的阶段，填补了国际上此项技术空白。相关论文“Large-scale production of functional human serum albumin from transgenic rice seeds ”(利用转基因水稻规模化生产重组人血清白蛋白)于2011年10月31日在线发表于《美国科学院院报
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