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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CD73 / NT5E Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/10.00 µg/20.00 µg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥18000.0 |
|---|---|---|---|
| 规格: | 10.00 µg | 产品价格: | ¥2310.0 |
| 规格: | 20.00 µg | 产品价格: | ¥3870.0 |
| 规格: | 50.00 µg | 产品价格: | ¥5170.0 |
| 规格: | 100.00 µg | 产品价格: | ¥11280.0 |
蛋白名称:Human CD73 / NT5E Protein (His Tag)
蛋白构建:A DNA sequence encoding the human NT5E (NP_002517.1) (Met1-Lys547) was expressed fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:1. Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) (Cat. 10904-H08H) bound MEDI9447 was 28 nM. 2. Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP). The specific activity is > 15,000 pmoles/min/μg.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Trp 27
蛋白分子量:The secreted recombinant human NT5E consists of 532 amino acids with the predicted molecular mass of 59.2 kDa.
蛋白NP号:NP_002517.1
蛋白氨基酸序列:Met1-Lys547
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
- 内容
- 询问日期
文献和实验2, Lyu S, et al. Identification of Phelligridin-Based Compounds as Novel Human CD73 Inhibitors.Journal of chemical information and modeling, PubMed ID: 33656342
-ChIP and X-ChIP. - http://www.abcam.com/index.html?pageconfig=resource&rid=10418&CFID=2422773&CFTOKEN=36329956 Expert protocols and tips for N-ChIP and X-ChIP. AbCAM Immunoprecipitation of Chromatin from Fixed Yeast Protocol PDF - http
(9): 1245–53. doi:10.1101/gr.6406307. PMID 17690206. ^ Bertone P, Stolc V, Royce TE, Rozowsky JS, Urban AE, Zhu X, Rinn JL, Tongprasit W, Samanta M, Weissman S, Gerstein M, Snyder M (December 2004). "Global identification of human transcribed sequences
X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏
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