- ￥2310 - 24150
- Sino Biological
- 北京
- 10171-H02H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human DLL4 Protein (Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/50.00 µg/100.00 µg/500.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥24150.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥2310.0 |
| 规格： | 100.00 µg | 产品价格： | ￥3870.0 |
| 规格： | 500.00 µg | 产品价格： | ￥9060.0 |
蛋白名称：Human DLL4 Protein (Fc Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Pro524) of human DLL4 (NP_061947.1) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized Mouse NOTCH1-His (Cat: 50654-M08B) at 2 μg/ml (100 μl/well) can bind human DLL4-hFc (Cat: 10171-H02H), the EC50 of human DLL4-hFc is 15-90 ng/mL. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1-8 µg/mL in the presence of 500 ng/mL recombinant human BMP2.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 27
蛋白分子量：The recombinant human DLL4/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 736 amino acids and predicts a molecular mass of 81 kDa. As a result of glycosylation, the rh DLL4/Fc monomer migrates as approximately 100-110 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_061947.1
蛋白氨基酸序列：Met1-Pro524
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Yasui Y, et al. In Vitro Differentiation of T Cell: From Human iPS Cells in Feeder-Free Condition.Methods in molecular biology (Clifton, N.J.), PubMed ID: 31396931
【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈，最后收集的蛋白质很有可能变性，或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域：E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域，仅由5个结构域组成，每个域均可以和IgG的Fc段结合，但不同的域结合强度略有差异。因此洗脱条件不均一，而且经常需要较低的pH值。如果减少串联结构域的个数，并且采取同型结构域串联，就可以避免不同结构域与抗体Fc 段亲和性的差异，洗脱条件会温和而均一。为此，我们可以做一个试验，比较一下
Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells
Single chain Fv (scFv) antibody fragments fused to the human immunoglobulin G (IgG) Fc moiety obtain IgG-like properties, but nevertheless they are still encoded by a single gene. As transient production of scFv-Fc proteins in mammalians
Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor
. In this chapter we describe our experience in the use of proteins containing selected regions of the mannose receptor fused to the Fc region of human IgG1 or murine IgG2b. Using these reagents we have discovered new and unexpected ligands for the mannose receptor
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