相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human FLT-3 / CD135 / FLK-2 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥22770.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥2310.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4520.0 |
蛋白名称:Human FLT-3 / CD135 / FLK-2 Protein (His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain of human FLT3 (P36888-1, with natural variant Thr 227 Met) (Met1-Asn541) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to bind biotinylated human FLT3L-His (Cat:10315-H07B) in a functional ELISA.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Asn 27
蛋白分子量:The recombinant human FLT3 consists of 526 amino acids and has a predicted molecular mass of 59.7 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 85-95 kDa due to glycosylation.
蛋白NP号:P36888-1
蛋白氨基酸序列:Met1-Asn541,T227M
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验上 Strep-tag 的 Fab 片段(Fab-Streps)特异性的结合到琼脂糖基质上。随后,加入全血或其他血液制剂流过柱子。目的细胞通过特异性的 Fab-Strep 作用从而结合到琼脂糖基质上,其他非目的细胞则有效的被洗去。最后,加入生物素使目的细胞从 Fab-Streps 上解离。至此,就已分离得到不带任何标记的、真实的目的细胞可用于下游的实验研究。 1. 实验流程 所需产品: CD3 Fab-TACS® Gravity Kit, human(IBA Lifesciences, Cat
Isolate or Deplete Human CD4+ T Cells - Whole Blood, Buffy Coat, MNC
/buffy coat to remove interfering soluble factors. (1)Dilute the whole blood or buffy coat in Buffer 1 (1 2). (2) Centrifuge at 600 x g for 10 min at 2-8°C. (3) Discard the plasma fraction/upper layer. Resuspend blood to the original
Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood
This chapter describes a robust method for the generation of iPS cells from non-cultured human cord blood cells. We describe the preparation of the CD34+ fraction from cord blood mononuclear cells, the protocols to determine the pluripotency
技术资料暂无技术资料 索取技术资料









