- ￥2310 - 25190
- Sino Biological
- 北京
- 10439-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Cystatin C / CST3 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/10.00 µg/20.00 µg/100.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥25190.0 |
|---|---|---|---|
| 规格： | 10.00 µg | 产品价格： | ￥2310.0 |
| 规格： | 20.00 µg | 产品价格： | ￥3870.0 |
| 规格： | 100.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：Human Cystatin C / CST3 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human Cystatin-C (NP_000090.1) (Ser 27-Ala 146) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC,R&D Systems, Catalog # ES009. The IC50 value is < 12 nM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 27
蛋白分子量：The mature recombinant human Cystatin-C consists of 131 amino acids and predicts a molecular mass of 14.8 kDa. The rh Cystatin-C migrates as approximately 17 kDa band in SDS-PAGE as a result of glycosylation.
蛋白NP号：NP_000090.1
蛋白氨基酸序列：Ser27-Ala146
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
Small Scale GST-Tag Purification Under Nature Conditions
4°C. d) ßME, DTT or DTE up to 10mM for proteins with many cysteines. e) 0.1-2% Triton X-100, NP40; or any other detergent that do not affect the biological activity of your protein. Sonicate in ice bucket 3 x 10sec or more if the cells
Purification of Human Multiprotein Complexes using OneSTrEP Technology
sufficient for visualising single protein bands by Coomassie Blue staining (Figure 1). The Strep -tag®II (SAWRHPQFGG) and its “double” sister, the One-STrEP-tag (tandem arrangement of Strep -tag®II, here called OneStrep), are reasonably small protein
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