Recombinant Human VTN / Vitronectin Protein (His Tag)
库存:
99
供应商:
北京义翘神州科技股份有限公司
规格:
50 µg/200 µg
规格:
50 µg
产品价格:
¥760.0
规格:
200 µg
产品价格:
¥2060.0
Vitronectin Protein, Human, Recombinant (His Tag): Product Information Purity≥95 % as determined by SDS-PAGE. Endotoxin< 10 EU per mg protein. ActivityMeasured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to Vitronectin-coated plates (10 μg/mL and 100 μL/well), approximately >40% cells will adhere specifically after 30 minutes at 37℃. Protein ConstructionA DNA sequence encoding the human VTN (NP_000629.3) (Met1-Leu478) was expressed with a polyhistidine tag at the C-terminus. Accession#NP_000629.3 Expressed HostHEK293 Cells SpeciesHuman Predicted N TerminalAsp 20 Molecule MassThe recombinant human VTN consists of 470 amino acids and predicts a molecular mass of 53.7 kDa. As a result of glycosylation, it migrates as an approximately 68.1 kDa band in SDS-PAGE under reducing conditions. FormulationLyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stability & StorageSamples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. ReconstitutionA hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Vitronectin Protein, Human, Recombinant (His Tag): Synonyms V75 Protein, Human; Vitronectin Protein, Human; VN Protein, Human; VNT Protein, Human Vitronectin Background Information Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin is a plasma glycoprotein implicated as a regulator of diverse physiological process, including blood coagulation, fibrinolysis, pericellular proteolysis, complement dependent immune responses, and cell attachment and spreading. Because of its ability to bind platelet glycoproteins and mediate platelet adhesion and aggregation at sites of vascular injury, vitronectin has become an important mediator in the pathogenesis of coronary atherosclerosis. As a multifunctional protein with a multiple binding domain, Vitronectin interacts with a variety of plasma and cell proteins. Vitronectin binds multiple ligands, including the soluble vitronectin receptor. It may be an independent predictor of adverse cardiovascular outcomes following acute stenting. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy. Full Name vitronectin
1, Han, Z; et al. Integrin αVβ1 regulates procollagen I production through a non-canonical transforming growth factor β signaling pathway in human hepatic stellate cells. The Biochemical journal, Pubmed: 33876829
2, Biasella, F; et al. Vitronectin and Its Interaction with PAI-1 Suggests a Functional Link to Vascular Changes in AMD Pathobiology. Cells
3, Lai, W; et al. A Protein Corona Adsorbed to a Bacterial Magnetosome Affects Its Cellular Uptake. Int J Nanomedicine, Pubmed: 32189964