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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human HER3 / ErbB3 Protein (ECD, His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
500 µg/50 µg
| 规格: | 500 µg | 产品价格: | ¥9750 |
|---|---|---|---|
| 规格: | 50 µg | 产品价格: | ¥1500 |
蛋白名称:Human HER3 / ErbB3 Protein (ECD, His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Thr 643) of human ErbB3 (NP_001973.2) precursor was expressed, with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. 2. Immobilized human ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 20
蛋白分子量:The recombinant human ErbB3 protein consists of 635 amino acids after the removal of signal peptide and has a calculated molecular mass of 70.2 kDa. As a result of glycosylation, the recombinant ErbB3 migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001973.2
蛋白氨基酸序列:Met1-Thr643
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Nilvebrant, J; et al. Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3. Cell. Mol. Life Sci., Pubmed: 23728098
3, Leitao, CD; et al. Evaluating the Therapeutic Efficacy of Mono- and Bivalent Affibody-Based Fusion Proteins Targeting HER3 in a Pancreatic Cancer Xenograft Model. Pharmaceutics, Pubmed: 32545760
4, Yu, F; Alesand, V; Nygren, PÅ. Site-Specific Photoconjugation of Beta-Lactamase Fragments to Monoclonal Antibodies Enables Sensitive Analyte Detection via Split-Enzyme Complementation. Biotechnol J, Pubmed: 29485240
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
Bait Protein Multivalent Complexes on Protein A Microbeads Human IgG (Jackson ImmunoResearch, cat. no. 009‐000‐003
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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