- ￥2570 - 21030
- Sino Biological
- 北京
- 10699-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD86 / B7-2 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/100.00 µg/200.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥21030.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥2570.0 |
| 规格： | 200.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：Human CD86 / B7-2 Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-His 239) of human B7-2 (NP_008820.2) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA . Immobilized human CD86 at 20 μg/ml (100 μl/well) can bind human CD28 with a linear range of 32-800 ng/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Leu 20
蛋白分子量：The recombinant human B7-2 consists of 231 amino acids and has a predicted molecular mass of 26.6 kDa. In SDS-PAGE, the apparent molecular mass of rhB7-2 is approximately 55-60 kDa due to glycosylation.
蛋白NP号：NP_008820.2
蛋白氨基酸序列：Met1-His239
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Pang X, et al. Cadonilimab, a tetravalent PD-1/CTLA-4 bispecific antibody with trans-binding and enhanced target binding avidity.mAbs, PubMed ID: 36872527
B7 家族 白细胞分化抗原及其功能 B7―1和B7-2 B7―1和B7―2分别称CD80和CD86，属B7家族成员。胞膜外区结构属IgSF成员，各有1个V样区和C2区，两者在氨基酸水平上有25％同源性。CD80胞质区较短，富含精氨酸，有1个RRES序列，为钙调蛋白依赖的磷酸化位点；CD86胞质区含有3个PKC磷酸化位点。 在活化的T细胞、B细胞和单核细胞表达水平明显增加。IL-4和TNF-7分别上调B细胞和单核细胞CD86表达
Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
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