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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human VEGF-C Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/5.00 µg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥15050.0 |
|---|---|---|---|
| 规格: | 5.00 µg | 产品价格: | ¥580.0 |
| 规格: | 50.00 µg | 产品价格: | ¥1780.0 |
| 规格: | 100.00 µg | 产品价格: | ¥2980.0 |
重组人 VEGF-C 蛋白 (His标签)(产品说明)
蛋白名称:Human VEGF-C Protein (His Tag)
蛋白构建:A DNA sequence encoding the mature form of human VEGFC (NP_005420.1) (Thr103-Arg227) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his (Cat:10542-H08H) at 2 μg/mL (100 μL/well) can bind VEGFR3 hFc(Cat:10806-H02H), the EC50 of VEGFR3 hFc(Cat:10806-H02H) is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Thr 103
蛋白分子量:The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.
蛋白NP号:NP_005420.1
蛋白氨基酸序列:Thr103-Arg227
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称:Human VEGF-C Protein (His Tag)
蛋白构建:A DNA sequence encoding the mature form of human VEGFC (NP_005420.1) (Thr103-Arg227) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his (Cat:10542-H08H) at 2 μg/mL (100 μL/well) can bind VEGFR3 hFc(Cat:10806-H02H), the EC50 of VEGFR3 hFc(Cat:10806-H02H) is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Thr 103
蛋白分子量:The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.
蛋白NP号:NP_005420.1
蛋白氨基酸序列:Thr103-Arg227
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验该产品被引用文献
1, Zhong, N; et al. Apatinib inhibits the growth of small cell lung cancer via a mechanism mediated by VEGF, PI3K/Akt and Ki-67/CD31. Journal of cellular and molecular medicine, PMID: 34590406
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重组人 VEGF-C蛋白 (His 标签)
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