ALK-1 蛋白|ALK-1 protein|ALK-1(Mouse, His & Fc Tag)

小鼠 ALK-1蛋白|ALK-1 protein|ALK-1(Mouse, C-human IgG1-Fc & His)（产品说明）
蛋白名称：ALK-1蛋白, ALK-1 protein
蛋白构建：A DNA sequence encoding the mouse ALK1 (NP_033742.2) precursor (Met 1-Pro 119) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is typically 60-300 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asp 23
蛋白分子量：The secreted recombinant mouse ALK1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 345 amino acids with a predicted molecular mass of 39 kDa. As a result of glycosylation, it migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_033742.2
蛋白氨基酸序列：Met1-Pro119
蛋白标签：C-human IgG1-Fc & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.