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Vimentin antibody

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • 2025年07月10日
  • WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, Sandwich ELISA
  • Rabbit
  • Human, Mouse, Rat, Cat, Dog
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat, Cat, Dog

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX100619

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, Sandwich ELISA

    • 浓度

      0.11 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      Vimentin

    • 抗体英文名

      Vimentin antibody

    • 抗体名

      Vimentin 抗体

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片2

    Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.
    Sample:Paraffin-Embedded adult mouse retina.
    Green: Vimentin protein stained by Vimentin antibody (GTX100619) diluted at 1:250.
    Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
    Blue: Fluoroshield with DAPI (GTX30920).

    产品细节图片3

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody (GTX100619) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    *The competitor is not affiliated with GeneTex and does not endorse this product.

    产品细节图片4

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

    产品细节图片5

    Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse colon.
    Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片6

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片7

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:20000.

    产品细节图片8

    Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse intestine.
    Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片9

    Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat testis by immunohistochemical analysis.
    Sample: Paraffin-embedded rat testis.
    Vimentin antibody (GTX100619) diluted at 1:500.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片10

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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    图标文献和实验
    该产品被引用文献
    Application Reference
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    相关实验
    • Discovery of Lamin B1 and Vimentin as Circulating Biomarkers for Early Hepatocellular Carcinoma

      electrophoresis (2DE) and MALDI-TOF/TOF mass spectrometry, our groups have identified lamin B1 (LMNB1) and vimentin (VIM) as promising biomarkers for detection of early HCC. Protein levels of both biomarkers were significantly elevated in cancerous tissues

    • Generation of Antibody Molecules Through Antibody Engineering

      been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional

    • The Antibody Molecule

      The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera

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    文献支持
    Vimentin antibody
    ¥1700 - 4000