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Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.
IgG
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Polyclonal
Unconjugated
Human, Mouse, Rat, Guinea pig, Pig, Mosquito
12 months from the shipping date of the product.
Human
GTX127375
Primary Antibodies
Available
GeneTex
Rabbit
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
0.14 mg/ml (Please refer to the vial label for the specific concentration.)
Cross-reactivity may occur with other LC3 isoforms
LC3B antibody
LC3B 抗体
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and LC3B knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% PFA at RT for 15 min.
Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with LC3B antibody (GTX127375) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody ) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
LC3B antibody detects MAP1LC3B protein by western blot analysis.
A. 20 μg Huh7 whole cell lysate/extract (untreated)
B. 20 μg Huh7 whole cell lysate/extract (3uM-Thapsigargin treatment for 12hr)
LC3B antibody (GTX127375) dilution: 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: LC3B stained by LC3B antibody (GTX127375) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.
LC3B antibody detects MAP1LC3B protein by western blot analysis.
A. 50 μg Rat brain lysate/extract
15% SDS-PAGE
LC3B antibody (GTX127375) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LC3B antibody (GTX127375) detects LC3B protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% PFA at 4oC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: LC3B antibody (GTX127375) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
LC3B antibody detects LC3B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
LC3B stained by LC3B antibody (GTX127375) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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