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- Thermo scientific -fermentas
- B30
- 2025年10月10日
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| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| B30 | 5 x 1 ml of each 10X buffer | B30 |
Tango™缓冲液用于常规限制酶介导的DNA双酶切 章节或浏览Fermentas公司网页DoubleDigest™ 搜索引擎(DoubleDigest™ )。
为确保酶切性能的稳定，Ferments公司的限制酶缓冲液含有BSA。BSA可增强限制酶的稳定性和结合DNA分离物中的杂质。缓冲液的多次冻-融不会导致BSA的沉淀。
Fermentas限制酶在推荐缓冲液中的酶活性达100%。但是有些限制酶需要添加剂才能达到100%活性，如AjuI、AlfI、BdaI、BplI、BseMII、FaqI、Eco57I、Eco57MI、Hin4I、TsoI需要添加剂S-adenosylmethionine(S-腺苷蛋氨基酸，随酶配套提供)。另外AarI和BveI需要添加剂寡核苷酸(随酶配套提供)；Esp3I需要添加剂DTT。
- 1X Buffer Tango™:
33 mM Tris-acetate (pH 7.5, 37°C), 10 mM Mg-acetate, 66 mM K-acetate, 0.1 mg/ml BSA - 1X Buffer R:
10 mM Tris HCl (pH 8.5, 37°C), 10 mM MgCl2 , 100 mM KCl, 0.1 mg/ml BSA - 1X Buffer O:
50 mM Tris-HCl (pH 7.5, 37°C), 10 mM MgCl2 , 100 mM NaCl, 0.1 mg/ml BSA - 1X Buffer G:
10 mM Tris-HCl (pH 7.5, 37°C), 10 mM MgCl2 , 50 mM NaCl, 0.1 mg/ml BSA - 1X Buffer B:
10 mM Tris-HCl (pH 7.5, 37°C), 10 mM MgCl2 , 0.1 mg/ml BSA
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文献和实验A Novel PCR-RFLP Detection Method Using an Optimized Set of Restriction Enzymes
, denaturing gradient gel electrophoresis (DGGE), heteroduplex analysis (HA), RNase A cleavage, chemical mismatch cleavage, Escherichia coli mismatch repair enzyme-based analysis (Grompe, 1993), and restriction endonuclease fingerprinting (Liu and Sommer, 1995
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
amount of restriction enzyme, in its respective buffer as recommended by the supplier, and at the optimal temperature for that specific enzyme. The optimal sodium chloride concentration in the reaction varies for different enzymes, and a set of three standard buffers
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