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- Thermo scientific -fermentas
- ER0961
- 2025年10月01日
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| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS | |
| 10X Buffer R | 10X Buffer Tango™ | ||||
| ER0961 | 200 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0961 | |
| ER0962 | 1000 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0962 | |
| Recommended buffer for 100% activity | Optimal temperature | Enzyme activity in Fermentas buffers, % | Tango™ buffer for double digestion | |||||
|---|---|---|---|---|---|---|---|---|
| B (blue) 1X |
G (green) 1X |
O (orange) 1X |
R (red) 1X |
Tango™ (yellow) 1X / 2X |
||||
| R | 37°C | 0-20 | 20-50 | 50-100 | 100 | 0-20 | 50-100 | 2X |
Lambda DNA
1.0%琼脂糖
46 切割位点
10 mM Tris-HCl (pH 8.5, 37°C), 10 mM MgCl2 , 100 mM KCl, 0.1 mg/ml BSA。
37°C酶切。
10 mM Tris-HCl (pH 7.5, 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA和50% (v/v)甘油。
Dcm: 无重叠 – 不影响。
CpG: 可能重叠 – 不影响。
EcoKI: 无重叠 – 不影响。
EcoBI: 可能重叠 – 影响不确定。
| Methylation type | Sequence | Cleavage effect |
|---|---|---|
| CpG | 5'...GAATGm5C G ...3' |
不阻止酶切 |
| CpG | 5'...m5C G AATGC...3' |
不阻止酶切 |
| bp from the recognition site to fragment end | ||||
|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 |
| 0 | 50-100 | |||
| Lambda | ΦX174 | M13mp18/19 | pBR322 | puc18/19 | pUC57 |
|---|---|---|---|---|---|
| 46 | 4 | 1 | 1 | 0 | 1 |
| pTZ19R/U | pTZ57R | pBluescriptIIKS(-/+) | pBluescriptIISK(-/+) | pACYC177 | pACYC184 |
| 0 | 1 | 0 | 0 | 2 | 3 |
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文献和实验Construction and Isolation of Recombinant MVA
Modified vaccinia virus Ankara (MVA) is a valuable tool for the expression of recombinant genes used for such purposes as the study of protein functions or characterization of cellular and humoral immune responses. A major advantage of MVA
Isolation of Recombinant MVA Using F13L Selection
Modified vaccinia virus Ankara (MVA) has become a widely used vector for vaccine and laboratory purposes. Despite significant advances in recombinant MVA technology, the isolation of recombinant viruses remains a tedious and difficult process
Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA
Modified vaccinia virus Ankara (MVA) is a highly attenuated and replication-deficient strain of vaccinia virus that is increasingly used as vector for expression of recombinant genes in the research laboratory and in biomedicine for vaccine
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