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|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS | |
| 10X FastDigest® Buffer | 10X FastDigest® Green Buffer | ||||
| FD0303 | 200 µl (200 react.) | 1.00 ml | 1.00 ml | FD0303 | |
| FD0304 | 400 µl (400 react.) | 1.00 ml | 1.00 ml | FD0304 | |
| Reaction temperature | Digestion time with 1µl of FastDigest® enzyme, min | bp from end of DNA required for complete digestion | Thermal inactivation | Incubation time without star activity, hours | |||
|---|---|---|---|---|---|---|---|
| Lambda, 1µg/20µl | Plasmid DNA, 1µg/20µl | PCR product, ~0.2µg/30µl | Genomic DNA, 1µg/10µl | ||||
| 37°C | 5 | 5 | 5 | 5 | 2 | No (chloroform extraction) |
16 |
Lambda DNA
1.0%琼脂糖
21 切割位点
1 µl FastDigest® EcoRV (Eco32I)可在
– 5分钟内酶切1 µg lambda DNA;
– 5分钟内酶切1 µg质粒DNA;
– 5分钟内酶切0.2 µg PCR扩增产物;
– 5分钟内酶切1 µg基因组DNA或30分钟内酶切5 µg基因组DNA。
Dcm: 无重叠 – 不影响。
CpG: 可能重叠 – 不影响。
EcoKI: 无重叠 – 不影响。
EcoBI: 可能重叠 – 影响不确定。
| Methylation type | Sequence | Cleavage effect |
|---|---|---|
| CpG | 5'...m5C G ATATm5C G ...3' |
不阻止酶切 |
| Lambda | ΦX174 | M13mp18/19 | pBR322 | puc18/19 | pUC57 |
|---|---|---|---|---|---|
| 21 | 0 | 0 | 1 | 0 | 1 |
| pTZ19R/U | pTZ57R | pBluescriptIIKS(-/+) | pBluescriptIISK(-/+) | pACYC177 | pACYC184 |
| 0 | 1 | 1 | 1 | 0 | 1 |
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文献和实验Universal RiboClone® cDNA Synthesis System
Universal RiboClone ® cDNA Synthesis System The Universal RiboClone ® cDNA Synthesis System contains the reagents required for the synthesis of double-stranded cDNA from mRNA
Universal RiboClone® cDNA Synthesis System
Universal RiboClone ® cDNA Synthesis System The Universal RiboClone ® cDNA Synthesis System contains the reagents required for the synthesis of double-stranded cDNA from mRNA
Figure 1―Analytical sensitivity of the Sequenom MassARRAY® System Figure 2―Somatic Mutation in FFPE Tumor Sample detected at 15% Frequency. Figure 2 shows the MassARRAY® spectrum for a KIT mutation, V559del
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