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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Eco32I (EcoRV) (10 U/µL)
- 保质期：
1年
- 供应商：
上海沪震实在有限公司
- 保存条件：
-20℃
- 规格：
2000units
Eco32I (EcoRV) (10 U/µL)
Enzyme : Eco32I (EcoRV)保存温度 : -20℃
货期 : 2-3天
Compatible Buffer : 10x Buffer R
Optimal Reaction Temperature : 37° C
Sensitive to Heat Inactivation: : Yes
Methylation Sensitivity : Not CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive
5' G A T ↓ A T C 3'
3' C T A ↑ T A G 5'
Thermo Scientific Eco32I (EcoRV) restriction enzyme recognizes GAT^ATC sites and cuts best at 37°C in R buffer (Isoschizomers: EcoRV). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
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文献和实验(note 7).Materials & Reagents ChIP lysis buffer 30ml final volume: 1.5 ml 1M Hepes 1.05ml 4M NaCl 3ml 10% Triton X (Tx-100) 300µl 10% NAD 24.15ml H20 ChIP high salt buffer 30ml final volume: 1.5ml 1M Hepes
上清。分装后一份待检测，其余冷冻备用。 6. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可 将标本放于 -20 ℃保存，但 应 避免反复冻融 . 7. 不能检测含NaN3的样品 ，因NaN3 抑制辣根过氧化物酶的（ HRP ）活性。 操作步骤 1. 标准品的稀释与加样：在酶标包被板上设标准品孔10 孔，在第一、第二孔中分别加标准品 100 μ l，然后在第一、第二孔中加标准品稀释液 50 μ l，混匀；然后从第一
Materials Purified Motor Protein, 20-80 µM Nucleotide Mix = 50 mM Mg・ATP gamma-32 P-ATP to give 5 000 - 10 000 cpm/nmol 10 mM HEPES, pH 7.2 1 mM EGTA 1 mM MgCl
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