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- Thermo scientific -fermentas
- ER0171
- 2025年09月24日
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| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS | |
| 10X Buffer Cfr9I | 10X Buffer Tango™ | ||||
| ER0171 | 300 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0171 | |
| ER0172 | 1500 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0172 | |
| Recommended buffer for 100% activity | Optimal temperature | Enzyme activity in Fermentas buffers, % | Tango™ buffer for double digestion | |||||
|---|---|---|---|---|---|---|---|---|
| B (blue) 1X |
G (green) 1X |
O (orange) 1X |
R (red) 1X |
Tango™ (yellow) 1X / 2X |
||||
| Cfr9I | 37°C | 0-20 | 0-20 | 0-20 | 0-20 | 20-50 | 0-20 | 1X |
Lambda DNA
0.7%琼脂糖
3 切割位点
10 mM Tris-HCl (pH 7.2, 37°C), 5 mM MgCl2 , 200 mM谷氨酸钠和0.1 mg/ml BSA。
37°C酶切。
10 mM Tris-HCl (pH 7.5, 25°C), 250 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA和50% (v/v)甘油。
备注
Dcm: 无重叠 – 不影响。
CpG: 可能重叠 – 部分影响。
EcoKI: 无重叠 – 不影响。
EcoBI: 无重叠 – 不影响。
| Methylation type | Sequence | Cleavage effect |
|---|---|---|
| CpG | CCCGGG |
部分影响 |
| bp from the recognition site to fragment end | ||||
|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 |
| 20-50 | 50-100 | |||
| Lambda | ΦX174 | M13mp18/19 | pBR322 | puc18/19 | pUC57 |
|---|---|---|---|---|---|
| 3 | 0 | 1 | 0 | 1 | 1 |
| pTZ19R/U | pTZ57R | pBluescriptIIKS(-/+) | pBluescriptIISK(-/+) | pACYC177 | pACYC184 |
| 1 | 1 | 1 | 1 | 1 | 0 |
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文献和实验Methylation analyis using restriction enzyme digestion
. Since in eukaryotic DNA (or actually in mammalian DNA) only cytosine in CG context can be methylated, the restriction enzymes with CG sequences within their restriction sites come in question. Two classical enzyme pairs are HpaII - MspI (CCGG) and SmaI - XmaI (CCCGGG
Methylated CpG Island Amplification
Protocol written by Minoru Toyota 1. Materials 1.1. MCA Restriction enzymes SmaI, XmaI T4 DNA ligase Taq DNA polymerase 10X PCR reaction buffer: 670mM Tris-HCl, pH 8.8 40mM MgCl2 160 mM NH
rAAV Vector Product Characterization and Stability Studies
has been reported by three groups. For human use, rAAV vectors are manufactured and tested in compliance with current Good Manufacturing Practices as outlined in the Code of Federal Regulations (21CFR) or European Good Manufacturing Practices (Eudralex
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