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- SM1841
- 2025年10月13日
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|
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SM1841 | 50 applic. (2x250 µl) | SM1841 | |
| SM1842 | 250 applic. (10x250 µl) | SM1842 |
- Features
-
- 条带窄，易辨认。
- 颜色稳定。
- 明亮的参考条带。
- 宽分子量范围。
- 即用型 一 与上样缓冲液预先混合，可直接凝胶上样。
- Applications
-
- 监控蛋白的SDS-聚丙烯酰胺凝胶电泳迁移。
- 监控Western杂交的蛋白转膜效率。
- SDS-聚丙烯酰胺凝胶电泳和Western杂交确定蛋白大小。
Spectra™ Multicolor Broad Range Protein Ladder是一个四色的分子量标准，含有10种蛋白，分子量范围为10 - 260 kDa。四种不同的发色团与蛋白结合产生明亮的彩色分子量条带，带型容易记忆。
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.
* Migration patterns were determined using respective NuPAGE® precast gels.
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文献和实验Fluorochrome Absorption and Emission Spectra
., RED613™ , EDC, PerCP), BD Cy-Chrome™ is a superior fluorochrome for third color analysis because of its high emission intensity and broad spectrum. As with our R-PE conjugates, an average of one BD Cy-Chrome™ molecule is coupled per antibody or protein
antibody with DIG (ChromaLink™ Digoxigenin). For IHC staining, cells or tissue sections are incubated first with unconjugated primary antibody against the first target protein followed by detection with antiprimary secondary antibody conjugated
Sample Protopcol for Two-Step Carbodiimide Coupling of Protein to MagPlex™ Magnetic Carboxylated Microspheres Microspheres should be protected from prolonged exposure to light throughout this procedure. 1.Resuspend
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