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文献和实验Addition of 3-A Overhangs (A-Tailing) to PCR Product
After amplification with a proofreading polymerase, place samples on ice and add 0.7-1 unit of Taq polymerase per tube directly into the PCR reaction tube. Mix well. Incubate at 72°C for 8-10 minutes. Place the reaction on ice
Ligation of DNA fragments by homopolymer tailing
Reagents10 × tailing buffer (1 M sodium cacodylate, pH 7.0, 10 mM CoCl2, 1 mM DTT)*Extreme care is required when making up the 10 × tailing buffer. Add the chemicals in the order stated to prevent precipitation.Stock solution (e.g. 20 mM
Applying the Marketing Mix (5 Ps) to Bionanotechnology
This chapter, based on concepts developed for my book, NanoInnovation (Tomczyk, Nanoinnovation: What Every Manager Needs to Know, 2011), is one of the first attempts to evaluate nanotechnology in the context of the “marketing mix” –
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