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充足
- 供应商:
Kapa biosystems
- 规格:
500rxn
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
DNA Repair and Solexa Library Construction 39. Repair DNA ends to generate blunt-ended DNA using the DNA End-Repair Kit (Epicentre). Combine: 1-34 µL of DNA (0.3 µg) 5 µL of 10X End-Repair
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
well in the past.Shearing volume should not exceed 200µlWash shearing orifices before and after each sample with the following settings:*All solutions must be filterd with a 0.2 micron filter before using.0.2 M HCl x20.2 M NaOH x3ddH2O x5Please use the Hydroshear manual pgs
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