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- Kapa biosystems
- 美国
- KK4809
- 2025年12月10日
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
) Phusion HF Master Mix (2X, Illumina) (see Step 45) Polymerase chain reaction (PCR) primers PE 1.0 and PE 2.0 (Illumina) (see Step 45) Primer mix for real-time PCR (see Step 38) Several companies offer primer synthesis service.
Multiplex Illumina Sequencing Using DNA Barcoding
cycler Gel electrophoresis apparatus (unit 2.5 ) Table 7.1.1 MaterialsOligonucleotide Sequences for Illumina Paired‐End Y‐Adapter and PCR Primers
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
from a premix of previously aliquotted stock solutions stored at -20degC (see Appendix B). To prepare the reaction premixes, reaction buffer is combined with the base-specific nucleotide mixes. Prior to use, the base-specific reaction premixes are thawed
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