- 询价
- Cell Signaling Technology
- 9145
- USA
- 2026年05月27日
- western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）,染色质免疫沉淀分析（ChIP）
- 兔
- 人,小鼠,大鼠,驴,仓鼠,细菌,猪,马
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb
- 是否单克隆：
1
- 适应物种：
人,小鼠,大鼠,驴,仓鼠,细菌,猪,马
- 应用范围：
western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）,染色质免疫沉淀分析（ChIP）
- 保存条件：
-20°c
- 抗原来源：
/
- 保质期：
详见说明书
- 供应商：
CST
- 抗原：
/
- 宿主：
兔
- 级别：
详见MSDS文件
- 库存：
大量
- 规格：
40 ul (8 western blots)/100 ul (20 western blots)/300 ul (60 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /40 ul (8 western blots)/100 ul (20 western blots)/300 ul (60 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥询价 | |
|---|---|---|---|
| 规格： | 40 ul (8 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (20 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (60 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 40 ul (8 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (20 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (60 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Jak/Stat Pathway
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145
PhosphoSitePlus ® protein, site, and accession data: STAT3
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IHC-F IF-IC F ChIP | H M R Mk (Hm) (B) (Pg) (Hr) | Endogenous | 79, 86 | Rabbit IgG |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey B=Bovine Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9145:
- ChIP Agarose , ChIP Magnetic , Flow , IHC / Frozen , IHC / Paraffin , Immunofluorescence* , Immunoprecipitation , Western Blotting
* Product-specific protocol.
Specificity / Sensitivity
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3.
Western Blotting
Western blot analysis of extracts from IFN-alpha treated Jurkat cells and HeLa cells (left), as well as EGF treated A431 cells (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb. Note that the basal phospho-Stat3 in A431 is detected by the antibody.
IHC-P (paraffin)
Immunohistochemical analysis using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb on SignalSlide (TM) Phospho-Stat 1/3/5 IHC Controls #8105 (paraffin-embedded 786-0 cells, untreated (left) or IFN-a treated (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Apc (min/+) mouse intestine, using Phosho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin embedded human breast carcinoma, specifically endothelial cells, untreated (left) or lambda phosphatase treated (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.
IHC-P (paraffin)
Immunnohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft using Phospho-Stat3 (Tyr705)(D3A7) XP® Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, IFN-alpha treated (left) or untreated (right), labeled with Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (green).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Background
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
- Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
- Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
- Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
- Bromberg, J.F. et al. (1999) Cell 98, 295-303.
- Darnell, J.E. et al. (1994) Science 264, 1415-21.
- Ihle, J.N. (1995) Nature 377, 591-4.
- Wen, Z. et al. (1995) Cell 82, 241-50.
- Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
Application References
- Tripathi, R.B. and McTigue, D.M. (2008) J Comp Neurol 510, 129-44. Applications: IC-IF
- Ernst, M.B. et al. (2009) J Neurosci 29, 11582-93. Applications: IHC-F (floating)
- Bauer, S. and Patterson, P.H. (2006) J Neurosci 26, 12089-99. Applications: IHC-FL (floating/frozen)
- Yao, Z. et al. (2010) Proc Natl Acad Sci U S A 107, 15535-40. Applications: Western Blotting
- Surdziel, E. et al. (2011) Blood 117, 4338-48. Applications: Western Blotting
- Stairs, D.B. et al. (2011) Cancer Cell 19, 470-83. Applications: IC-IF
- Anand, S. et al. (2011) Blood 118, 1610-21. Applications: Western Blotting
- Mainardi, M. et al. (2010) Proc Natl Acad Sci U S A 107, 16673-8. Applications: IF-F
- Matsuyama, H. et al. (2011) Blood 118, 6881-92. Applications: Western Blotting
- Ahluwalia, M. et al. (2010) J Thromb Haemost 8, 2252-61. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 9131 Phospho-Stat3 (Tyr705) Antibody
- 9132 Stat3 Antibody
- 9133 Stat3 Control Cell Extracts
- 9134 Phospho-Stat3 (Ser727) Antibody
- 9136 Phospho-Stat3 (Ser727) (6E4) Mouse mAb
- 9138 Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
- 9139 Stat3 (124H6) Mouse mAb
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1195 Phospho-Stat3 (Tyr705) Blocking Peptide
- 8105 SignalSlide® Phospho-Stat1/3/5 IHC Controls
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验稀释液的效果更好。4. 匹配的二抗和显色系统——「配一配和滴一滴」使用多聚 HRP（Poly-HRP）的二抗，即在二抗上连接大量 HRP，以充分放大信号；同时，配合优质的色原产品，提升灵敏度，增强信号。注：在人肺癌标本中使用 Sox2(D6D9) XP® RabbitmAb#3579 检测 Sox2 的表达，使用基于多聚-HRP 的 SignalStain® BoostIHCDetectionReagent 的检测灵敏度更高。注：DAB 色原比较：在人乳腺癌组织中使用 p-Stat3(Tyr705)(D3A7
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
used in accordance withmanufacturer recommendationsSignalStain?DAB Substrate Kit #8059:IHC analysis of paraffin-embedded human breastcarcinoma using Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb #9145. Chromogenicdetection was performed using #8059 (left
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