Eps15 Antibody

8855:

Immunoprecipitation , Western Blotting

Eps15 Antibody recognizes endogenous levels of total Eps15 protein. Based on sequence alignment, this antibody is predicted to cross-react with both Eps15a and Eps15b. This antibody does not cross-react with Eps15R.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Eps15 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using Eps15 Antibody.

Eps15 (EGFR pathway substrate 15) was originally discovered as a substrate for the kinase activity of EGFR (1). Eps15 has a tripartite structure comprising an amino terminal portion, which contains three evolutionary conserved EH protein-protein interaction domains, a central putative coiled-coil region required for constitutive oligmerization, and a carboxy terminal domain containing multiple copies of the amino acid triplet aspartate-proline-phenylalanine that constitute the AP2 binding domain. The carboxy terminal domain also contains two ubiquitin interaction motifs (UIMs), the last of which is indespensible for Eps15 binding to ubiquitin (1). Several lines of evidence support a role for Eps15 in clathrin-mediated endocytosis including the endocytosis of synaptic vesicles. Eps15 binds to AP-2 as well as other proteins involved in endocytosis and/or synaptic vesicle recycling, such as synaptojanin1 and epsin. Furthermore, Eps15 colocalizes with markers of the plasma membrane clathrin-coated pits and vesicles (2). Indeed, Eps15 has been shown to regulate the endosomal trafficking of c-Met (3) and EGFR (4), possibly through the recruitment of these ubiquitinated receptors to the rims of clathrin-coated pits by interacting with the ubiquitin tag through its UIMs. The EPS15 gene yields two isoforms that are believed to reside in distinct subcellular locations and thus implicated in different facets of endosomal trafficking (5). Human EPS15 has been mapped to chromosome 1p31-p32, a region displaying several non-random chromosomal abnormalities, including deletions in neuroblastoma and translocation in acute lymphoblastic and myeloid leukemias. Two translocations t(1;11)(p32;q11) were described in rare cases of myeloid leukemia, where the Eps15 gene was fused to the HRX gene resulting in two reciprocal fusion genes (6).

1. Fazioli, F. et al. (1993) Mol Cell Biol 13, 5814-28.
2. Tebar, F. et al. (1996) J Biol Chem 271, 28727-30.
3. Parachoniak, C.A. and Park, M. (2009) J Biol Chem 284, 8382-94.
4. Torrisi, M.R. et al. (1999) Mol Biol Cell 10, 417-34.
5. Roxrud, I. et al. (2008) J Cell Biol 180, 1205-18.
6. Bernard, O.A. et al. (1994) Oncogene 9, 1039-45.

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• 4796 Clathrin Heavy Chain (D3C6) XP^® Rabbit mAb
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9998 BSA
• 9999 Nonfat Dry Milk

For Research Use Only. Not For Use In Diagnostic Procedures.