- 询价
- Cell Signaling Technology
- 5676
- USA
- 2026年05月28日
- W, IP, IF-IC
- H,M,R,Hm,Mk
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- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
GSK-3α/β (D75D3) XP Rabbit mAb__®
- 抗原：
synthetic peptide surrounding Gln269 of human GSK-3α protein
- 应用范围：
W, IP, IF-IC
- 级别：
详见MSDS文件
- 供应商：
CST
- 保质期：
详见说明书
- 适应物种：
H,M,R,Hm,Mk
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M R Hm Mk | Endogenous | 51, 46 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | GSK-3α/β (D75D3) XP® Rabbit mAb detects endogenous levels of total GSK-3α and GSK-3β. The peptide sequence used as the antigen is 100% conserved between GSK-3α and GSK-3β in humans, monkeys, mice and rats. The antibody may prefer the conformation of GSK-3β by IF-IC. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Gln269 of human GSK-3α protein. Western Blotting
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® GSK-3α/β siRNA #6301, using GSK-3α/β (D75D3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). Western Blotting
Western blot analysis of extracts from GSK-3β (-/-) (lanes 1,2), GSK-3α (-/-) (lanes 3,4) and wild type (lanes 5,6) mouse embryonic fibroblast cells (MEF), untreated or insulin treated, using GSK-3α/β (D75D3) XP® Rabbit mAb. (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). IF-IC
Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® GSK-3α/β siRNA #6301 (right), using GSK-3α/β (D75D3) XP® Rabbit mAb (green). Actin filaments have been labeled wth DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3α regulates the production of amyloid-β peptides, a major component of the plaques that accumulate with progression of Alzheimer disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-β production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by γ-secretase, blocking accumulation of amyloid-β peptides in the brains of mice that overproduce APP (6).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
我的石蜡切片又「团灭」了！没关系，专业技术人员与您并肩推塔！
Antibody Diluent #8112 稀释PD-L2抗体（#82723）稀释度为1：200。11. 横沥封闭液，用卫生纸尽量吸干封闭液后，将一抗滴加在切片上。（此步建议一张张完成，防止干片）12. 将湿盒小心移入4度冰箱，过夜。CST技术人员正在配置和滴加抗体二抗孵育与显色13. 小心取出湿盒，在室温平衡45分钟左右。14. 洗涤：切片浸没于TBST中，3 ×5 min。15. 将二抗SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)#8114
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