- 询价
- Cell Signaling Technology
- 3876
- USA
- 2026年05月28日
- W, IP, IF-IC
- H,M,R,B
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- 文献和实验
- 技术资料
- 抗体英文名：
JARID1A (D28B10) XP Rabbit mAb__®
- 抗原：
synthetic peptide corresponding to the human JARID1A protein
- 应用范围：
W, IP, IF-IC
- 供应商：
CST
- 保质期：
详见说明书
- 适应物种：
H,M,R,B
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M (R) (B) | Endogenous | 200 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | JARID1A (D28B10) XP® Rabbit mAb detects endogenous levels of total JARID1A protein (both isoforms). The antibody does not cross-react with other JARID proteins, including JARID1B, JARID1C and JARID1D. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the human JARID1A protein. Western Blotting
Western blot analysis of extracts from various cell lines using JARID1A (D28B10) XP® Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of NTERA-2 cells using JARID1A (D28B10) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). |
| Background | The methylation state of lysine residues in histone proteins is a major determinant for formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The JARID (Jumonji/AT-rich interactive domain-containing protein) family contains four members: JARID1A (also RBP2 and RBBP2), JARID1B (also PLU-1), JARID1C (also SMCX) and JARID1D (also SMCY) (4). In addition to the JmJC domain, these proteins contain JmJN, BRIGHT, C5HC2 zinc-finger, and PHD domains, the latter of which binds to methylated histone H3 (Lys9) (4). All four JARID proteins demethylate di- and tri-methyl histone H3 Lys4; JARID1B also demethylates mono-methyl histone H3 Lys4 (5-7). JARID1A is a critical RB-interacting protein and is required for Polycomb-Repressive Complex 2 (PRC2)-mediated transcriptional repression during ES cell differentiation (8). A JARID1A-NUP98 gene fusion is associated with myeloid leukemia (9). JARID1B, which interacts with many proteins including c-Myc and HDAC4, may play a role in cell fate decisions by blocking terminal differentiation (10-12). JARID1B is over-expressed in many breast cancers and may act by repressing multiple tumor suppressor genes including BRCA1 and HOXA5 (13,14). JARID1C has been found in a complex with HDAC1, HDAC2, G9a and REST, which binds to and represses REST target genes in non-neuronal cells (7). JARID1C mutations are associated with X-linked mental retardation and epilepsy (15,16). JARID1D is largely uncharacterized.
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
我的石蜡切片又「团灭」了！没关系，专业技术人员与您并肩推塔！
Antibody Diluent #8112 稀释PD-L2抗体（#82723）稀释度为1：200。11. 横沥封闭液，用卫生纸尽量吸干封闭液后，将一抗滴加在切片上。（此步建议一张张完成，防止干片）12. 将湿盒小心移入4度冰箱，过夜。CST技术人员正在配置和滴加抗体二抗孵育与显色13. 小心取出湿盒，在室温平衡45分钟左右。14. 洗涤：切片浸没于TBST中，3 ×5 min。15. 将二抗SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)#8114
的染色强度。获得合适的染色强度后，将载玻片置于蒸馏水 (dH2O) 中以阻止进一步显色。抗体：Phospho–Stat3(Tyr705)(D3A7) XP® Rabbit mAb #9145样本：石蜡包埋的人类乳腺肿瘤显色检测：利用 SignalStain® DAB Substrate Kit #8059（左），竞争者提供的 DAB（中和右）CST 的 IHC/IF 明星产品推荐：生化试剂：Catalog NumberProduct NameProduct
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