- 询价
- Cell Signaling Technology
- 2956
- USA
- 2025年08月21日
- W, IHC-P, IF-IC, F
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
GFP (D5.1) XP Rabbit mAb__®
- 抗原：
synthetic peptide corresponding to the amino terminus of GFP
- 应用范围：
W, IHC-P, IF-IC, F
- 适应物种：
All
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | All | Transfected Only | 27 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | GFP (D5.1) XP® Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP. Western Blotting
Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP (D5.1) XP® Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected (left) or GFP-transfected (right), using GFP (D5.1) XP® Rabbit mAb. Flow Cytometry
Flow cytometric analysis of HCC827 cells, untransfected (blue) or GFP-transfected (green), using GFP (D5.1) XP® Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of COS-7 cells expressing a GFP-tagged fusion protein (green) using GFP (D5.1) XP® Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
肺癌病人的诊断和个性化治疗提供了有效的筛查工具。同样，以ROS1融合蛋白抗体（克隆号D4D6，货号#3287）为基础的IHC检测技术与断裂FISH方法相比，在非小细胞肺癌上IHC检测的准确度达到100%【5】。如此，我们期待检测ROS1融合变异的临床诊断用IHC试剂盒不久将成功推出。如要了解更多有关ALK和ROS1抗体的验证数据，请点击：ALK (D5F3) XP®Rabbit mAb #3633 和 ROS1 (D4D6) Rabbit mAb #3287。 本文
我的石蜡切片又「团灭」了！没关系，专业技术人员与您并肩推塔！
Antibody Diluent #8112 稀释PD-L2抗体（#82723）稀释度为1：200。11. 横沥封闭液，用卫生纸尽量吸干封闭液后，将一抗滴加在切片上。（此步建议一张张完成，防止干片）12. 将湿盒小心移入4度冰箱，过夜。CST技术人员正在配置和滴加抗体二抗孵育与显色13. 小心取出湿盒，在室温平衡45分钟左右。14. 洗涤：切片浸没于TBST中，3 ×5 min。15. 将二抗SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)#8114
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