- 询价
- Cell Signaling Technology
- 3590
- USA
- 2025年12月12日
- W, IP
- H,M,R,Mk,Pg
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-Rb (Ser780) (C84F6) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to resudues surrounding Ser780 of human Rb protein
- 应用范围：
W, IP
- 供应商：
CST
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Mk,Pg
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk (Pg) | Endogenous | 110 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Rb (Ser780) (C84F6) Rabbit mAb detects endogenous levels of total Rb protein only when phosphorylated at Ser780. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to resudues surrounding Ser780 of human Rb protein. Western Blotting
Western blot analysis of recombinant truncated Rb, without or with Rb blocking peptide, using Phospho-Rb (Ser780) (C84F6) Rabbit mAb. Western Blotting
Western blot analysis of extracts from COS and Jurkat cells using Phospho-Rb (Ser780) (C84F6) Rabbit mAb. IP
Immunoprecipitation of phospho-Rb (Ser780) from Jurkat cell lysates using Phospho-Rb (Ser780) (C84F6) Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input. |
| Background | The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit monclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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