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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Disposable gridcutter, 72 mm x 5 mm lanes. 48 rows, 1 column
- 供应商:
北京鼎国昌盛
- 规格:
10
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文献和实验only disposable homogenizers and vessels, and requiring" only one transfer of the DNA solution thereby reducing potential DNA cross contamination. The RAPD fingerprinting technique is thought to be sensitive to the quality of the DNA template (11).
BAC DNA Purification For Injection
Equilibrate tip-500 column with 10 ml QBT buffer (750 mM NaCl, 50mM MOPS pH 7.0, 15% isopropanol, 0.15% Triton X-100) Apply filtered DNA solution to the column Wash column twice with 30 ml QC buffer (1.0 M NaCl, 50 mM MOPS pH 7.0, 15% isopropanol
Preparation of Poly A+ RNA and Northern Analysis
(see below) with standards in the outside lanes. Run overnight at 35 V (constant). 20 X E Buffer Na2HPO4.7H2O 32.72 gm NaH2PO4.H2O 10.85 gm ddH2O to 1000 ml 1 x E/Formaldehyde Buffer ddH2O 773 ml 20 x E buffer 50 ml 37% formald. 177 ml
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