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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
8.8 cm flat spacer (8.3 cm actual length)
- 供应商:
北京鼎国昌盛
- 库存:
大量
- 规格:
2
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文献和实验. You may also need to adjust the AMPS/TEMED quantities used. You should aim to have the plug set in ~5 mins and the main g el after ~30 mins. 6) Immediately after the gel is poured, a flat 0.25mm spacer (or reversed shark tooth comb) should be placed into the acrylamide
Screening BAC filters with non-radioactive probes
) with 6 glass tubes (4cm in diameter; 24 cm in length). The probe DNA length should be more than 400 bp. If the DNA is shorter than 400 bp, the signal becomes too weak to detect. Disclaimer added by BACPAC Resources Center
Electrophoretic Mobility Shift Assay(EMSA)
20cm Gel Preparation, Sample Separation, and Visualization: Glass Plate Preparation:• Wash both panes of glass with windex and 70% EtOH• Dry with KimWipe• Assemble with toothed spacers on sides, winged spacer on bottom• Clamp corners
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