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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京鼎国昌盛
- 库存：
大量
- 英文名：
Disposable gridcutter, 2 mm x 7 mm lanes. 25 rows, 1 column
- 规格：
10
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文献和实验: each reaction contained 50 mM KCl, 10 mM Tris HCl (pH 8.3), " "2.0 mM MgCl 0.1 mM of each deoxynucleotide, 25 pmoles of a single 10 base " "primer OPA 04 (5'AGTCAGCCAC) (Operon Tech. Inc., Alameda, CA), 2% (v/v)" "glycerol, 1 unit DNA polymerase and 5 ng
Gene Knockout Techniques(1) The Knockout Process
among the progeny of a mutagenized population is a good assay to indicate that the mutagensis was successful. After 24 to 36 hours, set up 25 60mm plates with three mutagenized adults each. Typically we screen for twitchers before harvesting for the DNA prep
Preparation of Poly A+ RNA and Northern Analysis
(see below) with standards in the outside lanes. Run overnight at 35 V (constant). 20 X E Buffer Na2HPO4.7H2O 32.72 gm NaH2PO4.H2O 10.85 gm ddH2O to 1000 ml 1 x E/Formaldehyde Buffer ddH2O 773 ml 20 x E buffer 50 ml 37% formald. 177 ml
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