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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Delect Buffer Kit pH 7.3
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 规格:
1
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文献和实验Buffer P1 50 mM Tris-HCl pH 8.0 10 mM EDTA 100 μg/ml RNaseA The buffer and RNaseA can also be ordered from Qiagen separately (catalog numbers 19051 and 19101). Buffer P2 200 mM NaOH 1% SDS Buffer P3
in every 2-3 minutes. Important: Monitor the pH of the Gel/Binding buffer mixture after the gel completely dissolved. DNA yield will significantly decreased when pH > 8.0. If the color of the mixture become orange or red, Add 5 ul of 5M sodium acetate, pH 5.2
column into a clean 1.5 mL micro centrifuge tube. 10) Add 15-20 ul Elution Buffer(10mM Tris, pH 8.5) or water to the center of the membrane, incubate at room temperature for 3-5 minute. 11) Centrifuge at maximal speed ($13,000 x g
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