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- prospec
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- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
1.5ml Boil-Proof Microtubes with attached snap caps
- 库存：
大量
- 供应商：
北京鼎国昌盛
- 规格：
500
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文献和实验电镜样品制备方法 Specimen preparation for electron microscopy
into approx. 1mm cubes for fixing. This may be done in a drop of fix on a sheet of dental wax, using a razor blade. Place in glass processing vials and close with plastic caps. (Tissue may be stored at this stage.) Wash in 0.1M buffer - 1 hour x
attached cells with PBS without Ca2+/Mg2+(Prod. No. D8537) using 1-2ml for each 25cm2 of surface area. Retain the washings. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area. Rotate flask to cover
on a sheet of dental wax, using a razor blade. Place in glass processing vials and close with plastic caps. (Tissue may be stored at this stage.) Wash in 0.1M buffer - 1 hour x 2. (Or overnight at 4o C.) Post fix in osmium tetroxide in 0.2
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