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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 英文名:
Buffer Kit 2DGel DALT12
- 规格:
1
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
the spin cartridge into the same collection tube. 5) Repeat Steps 3–4 until the entire sample has been processed. 6) Add 700 μL Wash Buffer I to the spin cartridge. 7) Centrifuge at 12,000 × g for 15 seconds at room
) for 1 minute to elute DNA. 12) For genomic DNA clean up, repeat elution step 10-11 by adding another 15-20 ul Elution Buffer. 2. MicroElute™ DNA Clean-Up Vacuum/Spin Protocol Note: Please read through previous section before using
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