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- prospec
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- 以色列
- 2025年07月10日
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- 文献和实验
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- 库存：
大量
- 英文名：
Buffers and Wicks 2DGel FlatBed large
- 供应商：
北京鼎国昌盛
- 规格：
1
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
Recipes for the buffers are at the end of this protocol. 1.Label your protein with 32Pi.Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P. Neither nitrocellulose nor nylon
Immobilized pH gradient (IPG) as first dimension
strips were prepared by Pharmacia LKB Biotechnology AB and are commercially available. The strips were 3 mm wide and 180 mm long. In addition, the geometry of the immobilized pH gradient strips has been changed to allow the use of large sample application
Protein‐Protein Interactions Identified by Pull‐Down Experiments and Mass Spectrometry
spectrometry, pull?downs can be considered as the protein?based equivalent of a yeast two?hybrid screen. To improve the isolation of specific binding partners, pull?down methods are described involving the use of cross?linking, large?scale tissue lysates
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