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- 文献和实验
- 技术资料
- 保存条件：
-70C, RT
- 英文名：
Pico Methyl-Seq Library Prep Kit
- 供应商：
简石生物
HIGHLIGHTS
- All-inclusive kit for bisulfite conversion followed by Whole Genome Bisulfite Sequencing (WGBS) library preparation.
- Accommodates ultra-low DNA input and compatible with FFPE samples.
- Simple, ligation- and gel-free workflow can be completed in a few hours.
DESCRIPTION
The Pico Methyl-Seq Library Prep Kit provides a streamlined workflow for making WGBS libraries. Input DNA is randomly fragmented during the initial bisulfite treatment step followed by three rounds of amplification with uniquely designed primers. The procedure can accommodate as little as 10 pg input DNA (including that derived from FFPE samples), making it ideal for methylation analysis of precious, limited, and target-enriched samples.
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
) and construction of a library for sequencing. RELATED INFORMATION The advantages of next-generation sequencing have been described by Schones and Zhao (2008) . These platforms can be used for a variety of studies, including ChIP-Seq (Barski et al
RNA‐Seq: A Method for Comprehensive Transcriptome Analysis
. Protoc. Mol. Biol. 89:4.11.1?4.11.13. © 2010 by John Wiley & Sons, Inc. Keywords: RNA?Seq; transcriptome; high?throughput sequencing; gene expression; annotation; cDNA library preparation
RASL‐seq for Massively Parallel and Quantitative Analysis of Gene Expression
; selection; RASL?seq; bar?coding strategies; high?throughput screening GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library
