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- 技术资料
- 保存条件：
常温
- 保质期：
一年
- 英文名：
ZymoPURE II Plasmid Maxiprep Kit
- 供应商：
简石生物
HIGHLIGHTS
- Fastest: Simple 20 minute Maxipreps.
- Highest Yield: Purify up to 1.2mg of plasmid DNA directly from a spin-column.
- Ultra-Pure: EndoZero, vaccine grade, and transfection ready.
DESCRIPTION
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
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文献和实验Maxiprep preparation of Plasmid DNA
实验概要 The PureLink™ HiPure Plasmid DNA Maxiprep Kit allows purification of 500–850 μg of high-quality plasmid DNA from 100–200 mL overnight E. coli cultures in ~2 hours when cloning high copy number plasmids.
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
with occasional mixing. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II tightly capped when not in use.) 4. Add 250 ul ice-cold Buffer N3 and mix gently but throughly by inverting tube several times
Fastfilter Plasmid Maxi Kit Spin Protocol
Solution II, cover, and mix gently but throughly by inverting and rotating tube 10-15 times to obtain a cleared lysate. A 2 min incubation at room temperature may be necessary. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid
