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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
JIANSHI BIOTECH
- 供应商：
简石生物技术（浙江）有限公司
• Screen your recombinant colonies directly for protein production rather than for plasmid insert
• Easy way to prepare pure protein for small-scale studies
• Purified high-quality protein is suitable for enzyme kinetics, SDS-PAGE, MALDI-TOF, covalent modification, and
other applications
• No special instrumentation needed other than a benchtop microcentrifuge
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文献和实验of bacteria o.n. in LB + 100 mg/mL ampicillin. 2) Spin down 1.5 mL of each culture (5,000 rpm x 3 min.) Discard supernatant. Add additional 1.5 mL of cell suspension and spin a second time. 3) Resuspend pellet in 100 µL of H2O. 4) Add 300 µL
Yeast Miniprep from Robzyk and Kassir, Nucleic Acid Res. (1992) 20:3790 Materials : • 10 ml overnight culture yeast in drop out media (must be very dense) • STET (8% sucrose, 50 mM Tris pH 8, 50 mM EDTA, 5% Triton X
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
