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100 µg
蛋白名称:Lysozyme 2蛋白, Lysozyme 2 protein
蛋白构建:A DNA sequence encoding the human LYZL2 (Q7Z4W2-2)( Met 1-Ser148) was expressed with a C-terminal polyhistidine tag.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Lys 20
蛋白分子量:The secreted recombinant human LYZL2 consists of 139 amino acids and predicts a molecular mass of 16.2 KDa. The apparent molecular mass of the protein is approximately 19 KDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:Q7Z4W2-2
蛋白氨基酸序列:Met1-Ser148
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验药物6,,8)。另一方面,NZ-1对来自抗原的线性肽有很高的亲和性和特异性,于是我们开始研究是否能将NZ-1这些特性用于亲和标签系统中。 图1、3种不同长度的SPR和PA tag的亲和性比较将C末端上的3种长度的PA tag分别附加到模型蛋白T4 lysozyme(T4L)上,再注入已进行NZ-1固定化的传感芯片。分别加入3.125、6.65、12.5、25nM4种浓度的T4L,可以通过1:1 binding model进行曲线拟合求出解离常数(KD値)。曲线显示附加PA14或PA12时差别不大,虽然保持着非常慢
Anti-DYKDDDDK tag (L5) Affinity Gel
-tag fusion protein is in soluble form, this supernatant is ready for L5 column purification. If the DYKDDDDK-tag fusion protein is in an insoluble form, solubilize the inclusion body after centrifugation step (Step 6), refold the protein properly
His-tag protein purification using Ni-NTA magnetic beads
His-tag protein purification using Ni-NTA magnetic beads Materials and reagents Extraction buffer 20 mM Tris-HCl, pH 8.0 1 mM PMSF 1 mg/ml lysozyme 0.05
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