- ￥3870
- Sino Biological
- 50576-MNCE
- 2025年08月12日
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100 µg
蛋白名称：14-3-3 sigma蛋白, 14-3-3 sigma protein
蛋白构建：A DNA sequence encoding the mouse SFN (NP_061224.2) (Met 1-Ser 248) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminu.
表达宿主：E. coli
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：Please contact us for more information.
预测N端：Gly
蛋白分子量：The recombinant mouse SFN consists of 250 amino acids and has a calculated molecular mass of 27.7 kDa. It migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_061224.2
蛋白氨基酸序列：Met1-Ser248
蛋白标签：N-cleavage
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Basic Protocol 3: Secondary Screening and Analysis of Primary Hit Compounds of Proteasome‐Associated USP14 Materials AMC amine (Sigma
Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine
residues of ODC are known. Lysine 69 was recently identified as the residue in mouse ODC that forms a Schiff base with PLP and α-difluoromethyl-ornithine, an enzyme-activated irreversible inhibitor of ODC, forms adducts at both lysine 69 and cysteine 360
Thrombin Cleavage of GST-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of thrombin, temperature and length of incubation must be calibrated
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