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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human FcERI / FCER1A Protein (ECD,His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg/1 mg
| 规格: | 100 µg | 产品价格: | ¥3870 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥18110 |
蛋白名称:Human FcERI / FCER1A Protein (ECD,His Tag)
蛋白构建:A DNA sequence encoding the human FCER1A (P12319) (Met1-Gln205) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Val 26
蛋白分子量:The recombinant human FCER1A consists of 191 amino acids and predicts a molecular mass of 22.5 KDa. As a result of glycosylation, it migrates as an approximately 42-48 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号:P12319
蛋白氨基酸序列:Met1-Gln205
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
, H. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling. Cell 139:610‐622. Hudson, J.R., Jr., Dawson, E.P
Small Scale GST-Tag Purification Under Nature Conditions
of overloading). 3) Wash beads with 2x1.5ml PBS (washing: mix, spin 3min 3500rpm, keep supernatant aside). Keep sample of 40ul for PAGE-SDS of each washing . 4) Elute recombinant protein with 3 x 50ul elution buffer: 50mM TrisHCl pH8.0 10mM reduce
Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
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