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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse Oncostatin M / OSM Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/5.00 µg/20.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥22680.0 |
|---|---|---|---|
| 规格: | 5.00 µg | 产品价格: | ¥780.0 |
| 规格: | 20.00 µg | 产品价格: | ¥1580.0 |
| 规格: | 100.00 µg | 产品价格: | ¥3980.0 |
蛋白名称:Oncostatin M/OSM蛋白, Oncostatin M/OSM protein
蛋白构建:A DNA sequence encoding the mature form of mouse OSM (P53347) (Met 1-Arg 206) was expressed, with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to inhibit proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 50-200 ng/mL. 2. Measured in a cell proliferation assay using NIH3T3 cells. The ED50 for this effect is typically 2-12 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Asn 25
蛋白分子量:The secreted recombinant mouse OSM comprises 193 amino acids and has a calculated molecular mass of 22 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 35-40 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:P53347
蛋白氨基酸序列:Met1-Arg206
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Mouse Strains and Genetic Nomenclature
. Sci. U.S.A. 81:1189‐1192. Burgio, G., Szatanik, M., Guénet, J.‐L., Arnau, M.R., Panthier, J.J., and Montagutelli, X. 2007. Interspecific recombinant congenic strains
Array‐Based High‐Throughput Screening in Mouse Embryonic Stem Cells with shRNAs
.G., Hooper, M.L., Grandchamp, S., and Ephrussi, B. 1973. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. U.S.A. 70:3899‐3903. Brambrink, T
Comparative Protein Structure Modeling Using MODELLER
Figure 2.9.12 Typical errors in comparative modeling. (A ) Errors in side chain packing. The Trp 109 residue in the crystal structure of mouse cellular retinoic acid binding protein I (red) is compared
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