- 询价
- Cell Signaling Technology
- 3403
- USA
- 2025年07月12日
- W, IF-IC
- Rabbit
- H,M,R
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- 详细信息
- 技术资料
- 抗体英文名：
Myosin IIa Antibody
- 抗原：
synthetic peptide corresponding to the carboxy terminus of mouse myosin IIa
- 应用范围：
W, IF-IC
- 宿主：
Rabbit
- 适应物种：
H,M,R
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R | Endogenous | 230 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Myosin IIa Antibody detects endogenous levels of total myosin IIa protein. The antibody does not cross-react with the nonmuscle heavy chains of myosin IIb or IIc. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of mouse myosin IIa. Western Blotting
Western blot analysis of extracts from various cell types using Myosin IIa Antibody. Western Blotting
Western blot analysis of extracts from COS cells, mock transfected or transfected with nonmuscle EGFP-myosin IIa, IIb or IIc, using Myosin IIa Antibody. IF-IC
Confocal immunofluorescent analysis of HeLa cells using Myosin IIa Antibody (green) showing colocalization with actin filaments that have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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