FoxO1 (L27) Antibody

9454:

Western Blotting

FoxO1 (L27) Antibody detects endogenous levels of total FoxO1 protein and does not cross-react with endogenous FoxO3a or FoxO4. The antibody also cross-reacts with an unidentified protein at 95 kDa.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids within the amino terminus of mouse FoxO1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from IGROV-1 cells and untreated, λ phosphatase- or FBS-treated (20 min.) HeLa cells using FoxO1 (L27) Antibody.

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

1. Anderson, M.J. et al. (1998) Genomics 47, 187-199.
2. Galili, N. et al. (1993) Nat. Genet. 5, 230-235.
3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
4. Nakae, J. et al. (1999) J. Biol. Chem. 274, 15982-15985.
5. Rena, G. et al. (1999) J. Biol. Chem. 274, 17179-17183.
6. Guo, S. et al. (1999) J. Biol. Chem. 274, 17184-17192.
7. Seoane, J. et al. (2004) Cell 117, 211-223.
8. Arden, K.C. (2004) Mol. Cell 14, 416-418.
9. Yang, Y. et al. (2005) EMBO J. 24, 1021-1032.

• Eke, I. et al. (2010) J Clin Invest 120, 2516-27. Applications: Western Blotting

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• 9464 Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody
• 9461 Phospho-FoxO1 (Ser256) Antibody
• 2486 Phospho-FoxO1 (Ser319) Antibody
• 9465 Phospho-FoxO3a (Ser318/321) Antibody
• 9471 Phospho-FoxO4 (Ser193) Antibody
• 9472 FoxO4 Antibody
• 2499 FoxO4 (55D4) Rabbit mAb
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.