- 询价
- Cell Signaling Technology
- 7075
- USA
- 2025年09月12日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Anti-biotin, HRP-linked Antibody
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
1 ml/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 1 ml | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
Secondary Antibodies
Anti-biotin, HRP-linked Antibody #7075
Description
Affinity purified goat anti-biotin antibody is conjugated to horseradish peroxidase.
Applications
This product has been optimized to detect biotinylated protein markers.
Background
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
a simple ELISA method to detect human anti-antibody response.
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