- 询价
- Cell Signaling Technology
- 4882
- USA
- 2025年08月11日
- W, IP
- Rabbit
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
NF-κB2 p100/p52 Antibody
- 抗原：
synthetic peptide corresponding to residues at the amino-terminus of human p100NF-kappaB2
- 应用范围：
W, IP
- 宿主：
Rabbit
- 供应商：
CST
- 适应物种：
H,M,R,Mk
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 52 (mature). 120 (precursor). | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | NF-kappaB2 p100 Antibody detects endogenous levels of p100, the precursor, and p52, the mature form of NF-kappaB2. The antibody does not cross-react with other family members. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino-terminus of human p100NF-kappaB2. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from THP-1, L929, C6, and COS cells, using NF-kappaB2 p100 Antibody. |
| Background | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验【进展|热点】Nature Immunology：发现三个调控NF-kB非经典信号通路的microRNA
://www.nature.com/ni/journal/v11/n9/abs/ni.1918.html 内容简介： NF-κB是一组重要的转录因子，参与了诸多免疫反应过程，其中最为人熟知的就是在天然免疫中，NF-κB是最终启动炎症因子表达的因素之一。 NF-κB家族包括5个成员，即RelA(p65)、RelB、c-Rel、p105-p50(NF-κB1)和p100-p52(NF-κB2)，两两形成同源或者异源二聚体的形式发挥作用。通常情况下，NF-κB与其抑制蛋白IκB（包括IκBα、IκBβ
相关专题 NF-kappaB signaling pathway how the ubiquitination system regulates the activation of IKK-NF-κB signaling pathway NF-kappaB signaling pathway NF
激活－核转运检测试剂盒仅染色p65，不染色RelB、C-Rel、p50和p52。使用本试剂盒染色后NF-κB呈红色荧光，细胞核呈蓝色荧光.思考信号转导和检测的理论 我做了这样的回答(个人意见.大家可以讨论!)： 这种理论上来讲可行,但是实际操作可能拿不到你想要得结果,原因很简单,凭染色来判断是很难区分程度上的差异的,就比如做WB实验,经典的还是化学发光的方法,显色的方法只能得到信号比较强的蛋白,遇到信号比较弱的蛋白就误差很大了, 第二点,并不是所有能进核的蛋白都有结合DNA
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