- 询价
- Cell Signaling Technology
- 3672
- USA
- 2025年08月29日
- W
- Rabbit
- H,M,R,C,B,Pg
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- 详细信息
- 技术资料
- 抗体英文名：
Myosin Light Chain 2 Antibody
- 抗原：
synthetic peptide corresponding to amino-terminal residues of human Myosin Light Chain 2 (smooth muscle isoform)
- 应用范围：
W
- 宿主：
Rabbit
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H,M,R,C,B,Pg
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat C=Chicken B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R (C) (B) (Pg) | Endogenous | 18 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Myosin Light Chain 2 Antibody detects endogenous levels of total Myosin Light Chain 2 (smooth muscle). This antibody does not cross-react with the cardiac isoform of Myosin Light Chain 2, or Myosin Essential Light Chain. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human Myosin Light Chain 2 (smooth muscle isoform). Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HEK293, PL45, A431, C2C12 and C6 cells, using Myosin Light Chain 2 Antibody. |
| Background | Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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