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- Cell Signaling Technology
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- 2025年09月16日
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文献和实验Two dimensional peptide mapping
itself. Usually, 3-4 days exposure with a screen and flashed film is necessary to detect samples containing 100-200 cpm of 32P. Recipes for peptide mapping. It's sensible to make 2L
complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl
Blocking cDNA arrays printed on amine slides
so they can hybridize to the fluorescently labeled probe DNA . During the blocking step the array will disappear so be sure that the boundary of the array has been marked with a diamond pin as described in microarray construction. During the process, the exposed amine
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