HDAC6 (D2E5) Rabbit mAb

价格询价

品牌Cell Signaling Technology
货号
7558
产地USA
更新日期2026年05月28日

应用范围W, IP, IHC-P, IF-IC
适应物种H,Mk

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
HDAC6 (D2E5) Rabbit mAb
抗原：
recombinant protein specific to the carboxy terminus of human HDAC6 protein
应用范围：
W, IP, IHC-P, IF-IC
保质期：
详见说明书
级别：
详见MSDS文件
适应物种：
H,Mk
供应商：
CST
库存：
大量
是否单克隆：
1
保存条件：
-20°c
规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IF-IC	H Mk	Endogenous	160	Rabbit IgG

Protocols	7558: IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity	HDAC6 (D2E5) Rabbit mAb recognizes endogenous levels of total HDAC6 protein.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human HDAC6 protein. Western Blotting Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC6 (D2E5) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HDAC6 (D2E5) Rabbit mAb. IF-IC Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with MG132 (5 μM, 24 hr; right), using HDAC6 (D2E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).
Background	HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubule network (1). It is involved in the regulation of many cellular processes, including cell migration, immune synapse formation, viral infection, and degradation of misfolded proteins (1). HDAC6 contains two tandem catalytic domains that facilitate the deacetylation of multiple protein substrates, including histones and non-histone proteins such as tubulin, cortactin, and HSP90. Despite the ability to deacetylate histone proteins in vitro , there is no evidence for HDAC6-mediated deacetylation of histones in vivo (2,3). The acetylation/deacetylation of tubulin on Lys40 regulates binding and motility of the kinesin-1 motor protein and subsequent transport of cargo proteins such as JNK-interacting protein 1 (JIP1) (4). The acetylation/deacetylation of cortactin regulates cell motility by modulating the binding of cortactin to F-actin (5). Acetylation/deacetylation of HSP90 modulates chaperone complex activity by regulating the binding of an essential cochaperone protein, p23 (6,7). In addition to its role as a protein deacetylase, HDAC6 functions as a component of the aggresome, a proteinaceous inclusion body that forms in response to an accumulation of misfolded or partially denatured proteins (8). Formation of the aggresome is a protective response that sequesters cytotoxic protein aggregates for eventual autophagic clearance from the cell. HDAC6 contains a zinc finger ubiquitin-binding domain that binds both mono- and poly-ubiquitinated proteins (8). HDAC6 binds to both poly-ubiquitinated misfolded proteins and dynein motors, facilitating the transport of misfolded proteins to the aggresome (9,10). HDAC6 is also required for subsequent recruitment of the autophagic machinery and clearance of aggresomes from the cell (11). Thus, HDAC6 plays a key role in the protection against the deleterious effects of pathological protein aggregation that occurs in various diseases, such as neurodegenerative Huntington’s disease (11). Boyault, C. et al. (2007) Oncogene 26, 5468-76. Haggarty, S.J. et al. (2003) Proc Natl Acad Sci U S A 100, 4389-94. Zhang, Y. et al. (2003) EMBO J 22, 1168-79. Reed, N.A. et al. (2006) Curr Biol 16, 2166-72. Zhang, X. et al. (2007) Mol Cell 27, 197-213. Kovacs, J.J. et al. (2005) Mol Cell 18, 601-7. Murphy, P.J. et al. (2005) J Biol Chem 280, 33792-9. Seigneurin-Berny, D. et al. (2001) Mol Cell Biol 21, 8035-44. Kawaguchi, Y. et al. (2003) Cell 115, 727-38. Boyault, C. et al. (2006) EMBO J 25, 3357-66. Iwata, A. et al. (2005) J Biol Chem 280, 40282-92.
Application References	Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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